Optimization of gel formula from active fraction of manggost fruit (Garcinia mangostana. L) and antibacterial activity test against Staphylococcus epidermidis
Keywords:
Ethyl Acetate Fraction, Optimum Formula, Antibacterial, Simplex Lattice DesignAbstract
The purpose of this study was to determine the antibacterial activity of the ethyl acetate fraction of mangosteen peel and the effect of the combination of Carbopol, HPMC, and Propylene glycol as humectants on physical properties and antibacterial activity. The results of the fractionation of the extract were tested on Staphylococcus epidermidis bacteria using the diffusion method, the ethyl acetate fraction which had antibacterial activity with a concentration of 10% was made into a gel preparation with the composition of carbopol, HPMC, propylene glycol with run 14 obtained by the Simplex Latice Design method. The physical quality test of the preparation was carried out, namely organoleptic test, homogeneity, pH, adhesion, spreadability, viscosity, and antibacterial activity. Test data for physical quality and antibacterial activity analyzed using the Simplex Latice Design method, after which the analyzed using the Simplex Latice Design, and statistics using SPSS with the One Way Anova test. The results of the study stated that the ethyl acetate fraction of mangosteen peel can be made into mangosteen peel gel preparations for concentrations of Carbopol 94 1.5 g, HPMC 1000DB 2.5 g, propylene glycol 5 g with a spreadability value of 4.35 cm, viscosity 433.33, antibacterial 21, 4mm.
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