Optimization of serum formula active fraction of moringa leaf extract (Moringa oleifera Lambk) as antibacterial against Staphylococcus aureus
Keywords:
moringa leaves, staphylococcus aureus, antibacterial, simplex lattice design (SLD)Abstract
This study aims to determine the optimum formula for the serum active fraction of Moringa leaves with a combination of carbopol, NaCMC and propylene glycol as antibacterial. Material and methods the fractionation of the extract were tested on Staphylococcus aureus bacteria using the diffusion method. The ethyl acetate fraction which has antibacterial activity at a concentration of 15% was made into serum preparations with a combination of carbopol, NaCMC and propyleneglycol. Making serum formulas using the simplex lattice design method resulted in 14 serum formulas. Testing of the physical quality of the preparations carried out included organoleptic tests, homogeneity, pH, adhesion, viscosity, spreadability, and antibacterial activity. The results of the study on the ethyl acetate fraction of Moringa leaves with a concentration of 15% had antibacterial activity against S. aureus with an inhibition zone of 14.36 ± 1.1 mm. The TLC test the chemical compounds contained in the ethyl acetate fraction of Moringa leaves were flavonoids, saponins and tannins. The optimum combination proportions for serum preparations obtained variation values, namely carbopol 0.67% and NaCMC 3% and propylengliol 5.32%.
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References
Betina. Edisi ke III. Kares, V, 1972. “ Antibiotic” dalam Phermaceautical Applications of Thin layer and Paper Chromatography, Edisi ke III, Karel Macek (ed), 503-505, Elsevier Publishing Company, Amsterdam.
Bibah D, A. 2020. Uji Aktivitas Antibakteri sediaan Gel Ekstrak Daun kelor (Moringa oleifera Lambk.) terhadap bakteri Staphylococcus aureus ATCC 25923.
Choma., Irena, M., Edyta, M, G., 2010. Bioautography Detection in Thin Layer Chromatography. Journal of Chromatography.
Christianto, C.W., 2012. Efek Antibakteri Ekstrak Biji Alpukat (Persea Americana Mill) Terhadap pertumbuhan Streptococcus mutans, Oral Biol Dent., 4 (2) : 40-44.
Cowan, M. M. 1999. Plant Product as Antimicrobial Agent. J. Microbiology Reviews 12(4):564-582.
Damayanti, L. 2001. Uji Aktivitas Antibakteri tumbuhan Patikan Kebo (Euphorbia hirta L.) Terhadap Staphylococcus aureus ATCC 25923 Dan Escherichia coli ATCC 25922 Serta Profil kromatografi Lapis Tipisnya. Yogyakarta:Fakultas Farmasi universitas Gadjah Mada.
Davis, W. W., dan Stout, T.R., 1971. Disc Plate method of Microbiological Antibiotic Assay, Applied Microbiology, 22 (1) : 659-665.
Depkes RI. 2000. Parameter Standar umum Ekstrak tumbuhan Obat. Cetakan Pertama. Jakarta:Depkes RI. Halaman 10-11.
Draelos, Z.D., 2010. Cosmetic Dermatology Products and Prosedures. USA : Blackwell Publishing, Ltd.
Dunn, J. A., Robert, A. C., Richatd, T., Enas., Robert, J. G., 2008. Novel Topically Active Antimicrobial and Anti- Inflammatory Compounds For Acne. In: Walters, Kenneth A, editor. Dermatologic, Cosmoceutic And Cosmetic Development. New York: Informa Healthcare.h 243.
Efendi, Z., 2003. Peranan kulit Dalam Mengatasi terjadinya Acne Vulgaris Fakultas Kedokteran Universitas Sumatera Utara. Pp : 1-5.
Emad M. Abdallah. 2015. Antibacterial Properties of leaf Extracts of Moringa oleifera Lam. Growing in Sudan. SCIENCEDOMAIN International.
Emma, S. k., Iskandarsyah., Praptiwi., 2014. Evaluasi uji Stabilitas Sineresis Sediaan gel Yang Mengandung Monoksidil, Apigenin dan Perasan Herba Seledri ( Apium graveolens L). Jurnal Bul. Penelitian Kesehatan. Vol 4 : 213-222.
Ervizal; Rahayu, W.P; Wijaya, C.H;dan Sari, P.P. 2001. Aktivitas Antimikroba Ekstrak Kedawung (Parka roxburghii G. Don) Terhadap Bakteri Patogen (Online). Buletin Teknologi dan Industri Pangan. Vol XII no. 1. Dakses 5 oktober 2013.
Fatmawati N, Anwar E, Azizahwati. 2014. Formulasi Serum penghambat kerja Tirosinase yang Mengandung Fitosom Ekstrak Biji Lengkeng (Dimocarpus longan Lour) Menggunakan Eksipien Koproses Kasein- Xanthan Gum. FF UI.
Farooq F, Meenu r, Avinash T, Abdul AK, Shaila F. 2012. Medicinal Properties of Moringa oleifera: An Overview of Promising Healer, J Med Plants Res, 6(27): 4368-4373.
Granmayeh Rad, A., Abbasi, H., Afzali, M.H., 2011. Gold nanoparticles: Sythesising, Characterizing and Reviewing Novel Application in Recent Years. Physics Procedia 22, 203-203. http://doi.org/10.1016/j.phpro.2011.11.032
Harahap, Marwali. 2000. Ilmu Penyakit Kulit. Hipokrates:Jakarta.
Harborne, J. B. 1987. Metode Fitokimia Penuntun Cara modern Menganalisis Tumbuhan. Edisi kedua. Hal 5. 69-76. Diterjemahkan oleh Kosasih padmawinata dan Iwang Soedira. ITB Press. Bandung.
Hariana A., 2008. Tumbuhan Obat dan Khasiatnya Seri 2. Depok : Penebar Swadaya.
Hostettman, K., Hostettman, M., Maerston. 1995. Preparative Chromatography Technique : Application in Natural Product Isolation. (diterjemahkan Oleh Kosasih P) bandung: Penerbit ITB.
Ikalinus, Robertino, Widyastuti, Setiasih Eka. 2015. Skrining Fitokimia Ekstrak Etanol Kulit Batang kelor (Moringa oleifera). Bali: Universitas Udaya.
Kalpana, S., Moorthi, S. and kumara, S. 2013. Antimicrobial activity of difeferent extracts of leaf of Moringa oleifera (Lam) against gram positive and gram negative bacteria, International Journal of Current Microbiology, 12(2).
Khunaifi, M. 2010. Uji Aktivitas Antibakteri ekstrak daun binahong (Anredera Cordifolia (Ten.) Steenis) terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa. Jurusan biologi fakultas sains dan teknolohi. Skripsi. Malang. Universitas islam Negeri.
Krisnadi, a Dudi. 2015. Kelor Super Nutrisi. Maret 2015. http://kelorina.com/ebook.pdf. Diakses tanggal 29 September 2015.
Kusumaningtyas, E., Astuti, E., Darmono. 2008. Sensitivitas Metode Bioautografi Kontak dan Overlay dalam penentuan Senyawa Antikapang. Jurnal Ilmu Kefarmasian Indonesia, Vol. 6, No. 2. Hal 75-79.
Mitsui, T. 1997. New Cosmetic Science. Amsterdam: Elsevier Science B. V 28-32.
Moyo, b., Masika, P, and Muchenje, V. 2012. Antimicrobial activities of Moringa oleifera Lam leaf extracts. AFRICAN JOURNAL OF BIOTECHNOLOGY. Acamedic journals (Kenya), 11(11), pp. 2797-2802. Doi: 10.5897/AjB10. 686.
Mulyaningsih, S. 2004. Mikrobiologi dasar. Buku FMIPA UII. Yogyakarta.
Ojha, S., Sinha, S., Chaudhuri, S.D., Chadha, H., Jin, S.M., 2019. FORMULATION AND EVALUATION OF FACE SERUM CONTAINING BEE VENOM AND ALOE VERA GEL. World Journal of Pharmaceutical Research 8, 7.
Oluduro, A. O. 2012. Evaluation of Antimicrobial properties and nutritional potentials of Moringa oelifera Lam. Leaf in South-Western Nigeria. Malaysian Journal of Microbiology, 8(2), pp. 59-67.
Pratiwi, Sylvia T. 2008. Mikrobiologi farmasi. Jakarta : Erlangga.
Rocwood, J.L., Anderson, B.G., Casamatta, D.A. 2013. Potential Uses of Moringa oleifera and an Examination of Antibiotic Efficacy Conffered by Moringa oleifera Seed and Leaf Extrac using Crude Extraction Techniques. International Journal Of Phytotherapy Research vol.3.
Rollof, A., H. Weisgerber., U. Lang., B. Stimm. 2009. Moringa oleifera LAM. Weinheim:WILEY-VCH Verlag GmbH & Co. KgaA.
Rowe, R. c. (Ed.). 2009. Handbook of pharmaceutical excipients, 6. ed. Ed. APhA,(PhP) Pharmaceutical Press, London.
Singh, N., et al. 2012. Anti-Ulcer and Antioxidant Activity of Moringa oleifera (Lam) Leaves Against Aspirin and ethanol Induced Gastric ulcer in Rats. In International research Journal of Pharmaceuticals Vol 02. Hal 46-57.
Siti Rofida. 2020. Antibacterial and Characterization of Secondary Metabolite Compound from Ethyl Acetate and Ethanol Fraction of Leaves Moringa oliefera L.
Suriana, Neti., Shobariani Irni. 2013. Ensiklopedia Tanaman Obat. Malang:Rumah Ide.
Thakre, A.D. 2017. Formulation and Development of De Pigment Serum Incorporating Fruits Extract 2, 53.
Toripah. S, S., Abidjulu, J.,dan Wehantouw, F. 2014. Aktivitas Antioksidan dan Kandungan Total Fenolik Ekstrak Daun Kelor (Moringa oleifera Lambk). Manadoe:Program studi Farmasi FMIPA Universitas Samratulangi.
Unegbu, V., et al. 2020. Phytochemical and Antibacterial Properties of Moringa oleifera leaf extracts on Escherichia coli and Staphylococcus aureus.Nigerian Journal of Microbiology, 34(1):-5145-5152.
Utami P, Puspaningtyas DE. 2013. The Miracle of Herbs. Jakarta:Agro Media Pustaka.
Valgas, Cleidson, Simone Machado de Souza, Elza F A Smania, Artusmania Jr. 2006. Screening Methode to Determine Antibacterial Activity of Natural Products. Brazilian Journal Microbiologi.
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