Genetic alterations of CYP17A1 in the occurrence of colorectal cancer in Senegal

Anna Ndong; Bineta Keneme; Yacine Seye; Fatimata Mbaye; Mbacké Sembene

doi:10.53730/ijhs.v9n2.15594

Authors

Anna Ndong
anna.ndong@ucad.edu.sn
Cheikh Anta Diop University, Dakar, Senegal
Bineta Keneme Cheikh Anta Diop University, Animal Biology, Dakar-Fann, Senegal
Yacine Seye Cheikh Anta Diop University of Dakar (UCAD), DES, Residency in General Surgery
Fatimata Mbaye Cheikh Anta Diop University, Animal Biology, Dakar-Fann, Senegal
Mbacké Sembene Cheikh Anta Diop University, Animal Biology, Dakar-Fann, Senegal

Keywords:

Mutation, CYP17A1, c.-34T>C, Senegal, Colorectal cancer

Abstract

A cancer is said to be hormone-dependent when hormones influence the carcinogenesis process. Studies suggest a possible protective effect of hormone treatment in colorectal cancer. Given the hormone treatment, a thorough study of CYP17A1 is necessary. This study aims to assess the penetrance of CYP17A1 in the development of colorectal cancer. This study involved 24 colorectal cancer patients and 24 controls. For each sample, DNA extraction was performed, followed by CYP17A1 gene amplification and Sanger sequencing. The nature and position of mutations were identified using Mutation Surveyor version 5.1.2. DnaSP version 5.10, MEGA version 7.014, and the Arlequin program version 3.1 were used to highlight the parameters of variability, differentiation, and demogenetic evolution of the study population. The results revealed heterozygous mutations in the CYP17A1 gene and a substitution at the c.-34T>C promoter region. The absence of nonsynonymous mutations was revealed by very low genetic variability in the cancer population compared with controls. There was also little genetic differentiation and distance between the two populations. The frequent c.-34T>C mutation suggests that this polymorphism may modulate the transcriptional activity of CYP17A1 and consequently the hormones under this gene's control, and therefore colorectal tumor growth.

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